For manual or automated setup of protein crystallization screening conditions
- A ready-to-use kit format to which only protein needs to be added, for easy and fast screening
- 96 precisely defined chemical solutions containing ammonium sulfate as precipitant
- A precipitant that dehydrates the protein by competing with water molecules at its surface and thereby promoting the formation of macromolecular crystals
- A first set of solutions (conditions 1 - 48) containing AmSO4 in combination with 48 different salts known to be effective as co-crystallizers. This set allows the identification of suitable combinations of AmSO4 and salts to promote nucleation and crystal growth.
- A second set of solutions (conditions 49 - 72) containing different concentrations of AmSO4 in combination with various pH conditions in a 4 x 6 grid format to precisely define the effect of these factors.
- A third set (conditions 73 - 96) with the most popular solutions from the BMCD enabling screening for different combinations of co-precipitant, salts, and buffers that proved to be successful for other proteins.
- An intermediate step between results received from an AmSO4 containing suite (e.g., The Classics Suite) and before a narrower refinement
The AmSO4 Suite is available in 1.5 ml DWBlock and 10 ml tube formats.
No data available.