For manual or automated setup of protein crystallization screening conditions
The MPD Suite provides:
- A ready-to-use kit format to which only protein needs to be added, for easy and fast screening
- 96 precisely defined chemical solutions containing 2-methyl-2,4-pentandiol (MPD) as precipitant
- A precipitant that lowers the chemical activity of water and thereby reduces the number of electrostatic interactions between the protein and its solvent
- A precipitant that functions as cr yoprotectant and thereby protects cr ystals from breakage due to formation of crystalline ice when performing flash-freezing experiments
- A first set of solutions (conditions 1–48) containing a combination of MPD with 48 different salts known to be effective as co-crystallizers
- A second set of solutions (conditions 49–72) containing different concentrations of MPD in combination with various pH conditions in a 4x6 grid format to precisely define the effect of these factors
- A third set (conditions 73–96) with the most popular solutions from the BMCD enabling screening for different combinations of co-precipitant, salts, and buffers that proved to be successful for other proteins
- An intermediate step between results received from an MPD containing suite (e.g., The Classics Suite), and before a narrower refinement
The MPD Suite is available in 1.5 ml DWBlock and 10 ml tube formats.
No data available.