For manual or automated setup of protein crystallization screening conditions
The PEGs Suite provides:
- A ready-to-use kit format to which only protein needs to be added, for easy and fast screening
- 96 precisely defined chemical solutions containing polyethylene glycol polymers as precipitant
- Solutions with polyethylene glycol of different molecular weight
- The promotion of protein-protein interactions through molecular crowding
- A first set of solutions (conditions 1-48) containing PEG in a wide range of molecular weights (200 up to 20,000), set at various concentrations and four different pH values
- A second set of solutions (conditions 49-96) containing PEG 3350 together with a wide spectrum of low concentration salts to analyze the effects of the PEG-salt combination on the protein
- The possibility to run the two sets of solutions after each other and get more information by adjusting the pH of the second set accordingly
- An intermediate step between results received from a PEG containing suite (e.g., The Classics Suite) and before a narrower refinement
The PEGs Suite is available in 1.5 ml DWBlock and 10 ml tube formats.
Citation(s):
- Shaw K et al. 2009 Anal Chim Acta 652 231–3
- Mauk M et al. 2014 Methods Mol Biology 1256 15–40
- Lim J et al. 2018 J Assist Reprod Gen 35 637–41
- Zhang L et al. 2015 Biomicrofluidics 9 064118
- Miyaoka Y et al. 2016 Sci Rep-uk 6 23549
- Takamura Y et al. PLoS ONE 13 e0201851
No data available.